Reducing the stresses placed upon the oocyte during egg retrieval and handling process is crucial to maintain the fertilization and developmental capacity of the female gamete. A series of products particular to these applications are available to ensure optimal results.
The oocyte retrieval may require a buffered media to be used to retrieve oocytes from ovarian follicles at time of oocyte pick-up. Follicular aspirates are commonly added to a buffered media to allow for oocyte identification and manipulation outside of the laboratory incubator.
A rinsing medium is used to physically wash the cumulus-oocyte complex at the room’s atmosphere in preparation for denudation for the Intra-Cytoplasmic Sperm Injection (ICSI), oocyte vitrification, or conventional insemination for in vitro fertilization. A buffered medium is essential to maintain homeostasis during this process.
Cumulus cells surrounding the oocyte must be removed prior to oocyte micromanipulation and vitrification. The ability to quickly and effectively do this decreases the possibility of stresses imposed to the oocyte from this process.
Vitrification is an ultra-rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step-wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra-rapid cooling of the oocyte when plunged into liquid nitrogen — eliminating concerns of damaging ice-crystal formation associated with traditional slow-freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.
Irvine Scientific’s vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.