Processes and Protocols

Explore a wide range of procedures and proven protocols in embryo development and let us help you improve your results while simplifying your processes.

To achieve the best possible outcomes, sperm processing should include different techniques that are tailored for the variability of semen samples. Several processes are available to address the diverse differences in sperm quality, including a simple wash, swim-up, and density gradient sperm separation. Irvine Scientific's sperm processing media can be used for each laboratory's customized protocols for various types of sperm. Our andrology media also includes a variety of sperm preservation media for short or long-term storage of sperm.


Density gradient separation is one of the most common sperm preparation techniques, where concentrated sperm with the best motility are separated from other debris (poor or non-motile sperm, white blood cells, etc.) by using a colloidal solution of different densities.


Sperm washing is a technique used to remove sperm from the semen. This process uses media that provides a buffered environment that is able to support sperm motility and vitality which is required for successful fertilization.


Sperm freezing has become a routine process that depends greatly on the survivability and usability of the sperm after the preservation and thawing process. Sperm cryopreservation media protects sperm from the damaging effects of ice-crystal formation during the freezing process and subsequent long-term storage.


The special design and mode of filling and sealing of the CBS™ High Security straws for cryopreservation of human biological samples makes the straws fully hermetic in ultra-low temperatures. Manufactured from biocompatible materials, the straws are used in medically-assisted procreation techniques, particularly for human gamete and embryo preservation.


Reducing the stresses placed upon the oocyte during egg retrieval and handling process is crucial to maintain the fertilization and developmental capacity of the female gamete. A series of products particular to these applications are available to ensure optimal results.


The oocyte retrieval may require a buffered media to be used to retrieve oocytes from ovarian follicles at time of oocyte pick-up. Follicular aspirates are commonly added to a buffered media to allow for oocyte identification and manipulation outside of the laboratory incubator.


A rinsing medium is used to physically wash the cumulus-oocyte complex at the room’s atmosphere in preparation for denudation for the Intra-Cytoplasmic Sperm Injection (ICSI), oocyte vitrification, or conventional insemination for in vitro fertilization. A buffered medium is essential to maintain homeostasis during this process.


Cumulus cells surrounding the oocyte must be removed prior to oocyte micromanipulation and vitrification. The ability to quickly and effectively do this decreases the possibility of stresses imposed to the oocyte from this process.


Vitrification is an ultra-rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step-wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra-rapid cooling of the oocyte when plunged into liquid nitrogen — eliminating concerns of damaging ice-crystal formation associated with traditional slow-freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.

Irvine Scientific’s vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.


Fertilization in vitro requires an environment that supports biological processes to achieve successful and normal fertilization events. In some cases, the micromanipulation of gametes may be required to circumvent conventional insemination practices.


Cryopreservation is preserving supernumerary embryos at sub-zero temperatures for use in subsequent treatment cycles. The long term storage of precious reproductive material provides opportunities to preserve reproductive potential for the future.


Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step‐wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra‐rapid cooling of the oocyte when plunged into liquid nitrogen — eliminating concerns of damaging ice‐crystal formation associated with traditional slow‐freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.

Irvine Scientific’s vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.


Successful embryo cryopreservation is an important step for IVF laboratories to maximize a patient's IVF cycle. Irvine Scientific provides cryopreservation media for conventional slow-freezing and thawing of cleavage and blastocyst stage embryos.


Our preservation media for human sperm produces the maximum survival of sperm, including yolk-based TYB for freezing or refrigeration, which is only offered by Irvine Scientific.


The embryo transfer is a simple, but critical, step at the completion of the in vitro fertilization process with the objective to facilitate a healthy conception and pregnancy.


The embryo transfer is a simple, but critical, step at the completion of the in vitro fertilization process with the objective to facilitate a healthy conception and pregnancy.